![]() 31 and 49 identified metabolites were exclusively detected in SSM and NSSM, respectively, which mainly belong to carboxylic acids and derivatives, polyketides and fatty acyls. KEGG analysis showed the pathway of linoleic acid metabolism, and glyoxylate and dicarboxylate metabolism were mainly enriched. 287 differential metabolites were screened including 112 up-regulated and 175 down-regulated and they belong to lipids and lipid-like molecules, and phenylpropanoid and polyketides. ![]() 10,108 substance peaks had been detected altogether, and 4759 metabolites had been identified from negative and positive ion model. Analysis indicated distinct differences between sweated and non-sweated samples. Multivariate analysis was conducted to screen differential metabolites. An untargeted UPLC/MS-based metabolomics was performed to discover metabolites profile variation of S. ![]() Due to the complexity of TCM, changes in metabolites should be investigated metabolome-wide after “ Sweating”. miltiorrhiza showed a significant difference after “ Sweating”. Previously, chromatogram of 10 characteristic metabolites in S. “ Sweating”, a processing method of Traditional Chinese Medicine (TCM), results in great changes in pharmacology and pharmacodynamics. ![]() Salvia miltiorrhiza has numerous compounds with extensive clinical application.
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